Legends: 1, ACT_SITE Proton acceptor. {ECO:0000256|HAMAP- Rule:MF_00934}; 2, BINDING S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_00934}; 3, BINDING S-adenosyl-L-methionine; via carbonyl oxygen. {ECO:0000256|HAMAP- Rule:MF_00934}; 4, SITE Interaction with substrate rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}; 5, REGION S-adenosyl-L-methionine binding. {ECO:0000256|HAMAP-Rule:MF_00934}; 6, ipat:N6_MTASE [T].
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ID Q5E1W3_VIBF1 Unreviewed; 279 AA.
AC Q5E1W3;
DT 15-MAR-2005, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2005, sequence version 1.
DT 10-MAY-2017, entry version 63.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN Name=yhiR {ECO:0000313|EMBL:AAW86983.1};
GN Synonyms=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN OrderedLocusNames=VF_2488 {ECO:0000313|EMBL:AAW86983.1};
OS Vibrio fischeri (strain ATCC 700601 / ES114).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC Vibrionaceae; Aliivibrio.
OX NCBI_TaxID=312309 {ECO:0000313|EMBL:AAW86983.1, ECO:0000313|Proteomes:UP000000537};
RN [1] {ECO:0000313|EMBL:AAW86983.1, ECO:0000313|Proteomes:UP000000537}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700601 / ES114 {ECO:0000313|Proteomes:UP000000537};
RX PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium
RT with pathogenic congeners.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC Rule:MF_00934}.
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DR EMBL; CP000020; AAW86983.1; -; Genomic_DNA.
DR RefSeq; WP_011262845.1; NC_006840.2.
DR RefSeq; YP_205871.1; NC_006840.2.
DR ProteinModelPortal; Q5E1W3; -.
DR STRING; 312309.VF_2488; -.
DR EnsemblBacteria; AAW86983; AAW86983; VF_2488.
DR GeneID; 3279597; -.
DR KEGG; vfi:VF_2488; -.
DR PATRIC; 20115760; VBIVibFis127983_2518.
DR eggNOG; ENOG4105D6S; Bacteria.
DR eggNOG; COG2961; LUCA.
DR HOGENOM; HOG000262479; -.
DR KO; K07115; -.
DR OMA; TYAIWYP; -.
DR OrthoDB; POG091H04AQ; -.
DR Proteomes; UP000000537; Chromosome I.
DR GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-HAMAP.
DR HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR007473; RlmJ.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR Pfam; PF04378; RsmJ; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000000537};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW Reference proteome {ECO:0000313|Proteomes:UP000000537};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT REGION 143 144 S-adenosyl-L-methionine binding.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT ACT_SITE 164 164 Proton acceptor. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 19 19 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 42 42 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 100 100 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 118 118 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 164 164 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT SITE 4 4 Interaction with substrate rRNA.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 2 266 ihamap:23SrRNA_methyltr_J [T]
FT MYHIT 241 247 ipat:N6_MTASE [T]
FT MYHIT 33 277 ipfam:RsmJ [T]
SQ SEQUENCE 279 AA; 32201 MW; BB54B461B087DED6 CRC64;
MLSYRHSFHA GNHADVVKHI VQSLILNSLT QKDKPFVYHD THSGVGRYDL THEWSEKTGE
YKQGIARIWD QANLPEEITS YLDAIKTLND GEELRYYPGS PRVGRAHLRR QDRMVLTELH
PSDYPLLEQE FHRDRQVSIY KEDGFARLKA SLPPKERRGL VLIDPPYELA KEYRDVVTAI
GQSHKRWATG IYAIWYPVVN RHDIDDMING LKKLDIRNIL QIELGVSPDT NERGMTASGM
IVINPPWKLE SQMNDILPFL KEAIAPATGH FKVEWIVPE
//
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