Entry bact:HYCA_ECOLI

ID   HYCA_ECOLI              Reviewed;         153 AA.
AC   P0AEV4; P16427; Q2MAA4;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   15-FEB-2017, entry version 71.
DE   RecName: Full=Formate hydrogenlyase regulatory protein HycA;
GN   Name=hycA; Synonyms=hevA; OrderedLocusNames=b2725, JW2695;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=2187144; DOI=10.1111/j.1365-2958.1990.tb00590.x;
RA   Boehm R., Sauter M., Boeck A.;
RT   "Nucleotide sequence and expression of an operon in Escherichia coli
RT   coding for formate hydrogenlyase components.";
RL   Mol. Microbiol. 4:231-243(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION.
RX   PubMed=1625581; DOI=10.1111/j.1365-2958.1992.tb00873.x;
RA   Sauter M., Boehm R., Boeck A.;
RT   "Mutational analysis of the operon (hyc) determining hydrogenase 3
RT   formation in Escherichia coli.";
RL   Mol. Microbiol. 6:1523-1532(1992).
RN   [5]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R.,
RA   Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
RN   [6]
RP   FUNCTION, REGULON, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=12426353; DOI=10.1128/JB.184.23.6642-6653.2002;
RA   Skibinski D.A., Golby P., Chang Y.S., Sargent F., Hoffman R.,
RA   Harper R., Guest J.R., Attwood M.M., Berks B.C., Andrews S.C.;
RT   "Regulation of the hydrogenase-4 operon of Escherichia coli by the
RT   sigma(54)-dependent transcriptional activators FhlA and HyfR.";
RL   J. Bacteriol. 184:6642-6653(2002).
CC   -!- FUNCTION: Regulatory protein for the formate hydrogenlyase system.
CC       Could act by directly interacting with FhlA or by preventing the
CC       binding of FhlA to the upstream activatory sequence
CC       (PubMed:1625581). Also down-regulates expression of the hyf operon
CC       (PubMed:12426353). {ECO:0000269|PubMed:12426353,
CC       ECO:0000269|PubMed:1625581}.
CC   -!- DISRUPTION PHENOTYPE: Increased expression of the hyf operon
CC       (PubMed:12426353). {ECO:0000269|PubMed:12426353}.
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DR   EMBL; X17506; CAA35546.1; -; Genomic_DNA.
DR   EMBL; U29579; AAA69235.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75767.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76802.1; -; Genomic_DNA.
DR   PIR; S08619; S08619.
DR   RefSeq; NP_417205.1; NC_000913.3.
DR   RefSeq; WP_000158056.1; NZ_LN832404.1.
DR   ProteinModelPortal; P0AEV4; -.
DR   BioGrid; 4262102; 11.
DR   IntAct; P0AEV4; 1.
DR   STRING; 511145.b2725; -.
DR   PaxDb; P0AEV4; -.
DR   PRIDE; P0AEV4; -.
DR   EnsemblBacteria; AAC75767; AAC75767; b2725.
DR   EnsemblBacteria; BAE76802; BAE76802; BAE76802.
DR   GeneID; 947193; -.
DR   KEGG; ecj:JW2695; -.
DR   KEGG; eco:b2725; -.
DR   PATRIC; 32120850; VBIEscCol129921_2817.
DR   EchoBASE; EB0469; -.
DR   EcoGene; EG10474; hycA.
DR   eggNOG; ENOG4108VRC; Bacteria.
DR   eggNOG; ENOG4111JXQ; LUCA.
DR   HOGENOM; HOG000124058; -.
DR   KO; K15833; -.
DR   OMA; HAMSCAA; -.
DR   BioCyc; EcoCyc:EG10474-MONOMER; -.
DR   PRO; PR:P0AEV4; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   InterPro; IPR021285; Tscrpt_reg_HycA.
DR   Pfam; PF11046; HycA_repressor; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    153       Formate hydrogenlyase regulatory protein
FT                                HycA.
FT                                /FTId=PRO_0000084100.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT         1    145       ipfam:HycA_repressor [T]
SQ   SEQUENCE   153 AA;  17627 MW;  CB61EC856450A0AD CRC64;
     MTIWEISEKA DYIAQRHRRL QDQWHIYCNS LVQGITLSKA RLHHAMSCAP DKELCFVLFE
     HFRIYVTLAD GFNSHTIEYY VETKDGEDKQ RIAQAQLSID GMIDGKVNIR DREQVLEHYL
     EKIAGVYDSL YTAIENNVPV NLSQLVKGQS PAA
//