ID A2BNB4_HYPBU Unreviewed; 441 AA.
AC A2BNB4;
DT 20-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 20-FEB-2007, sequence version 1.
DT 10-MAY-2017, entry version 57.
DE RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01893};
DE AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
DE Short=Psi54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
GN Name=pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
GN OrderedLocusNames=Hbut_1661 {ECO:0000313|EMBL:ABM81475.1};
OS Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Pyrodictiaceae; Hyperthermus.
OX NCBI_TaxID=415426 {ECO:0000313|EMBL:ABM81475.1, ECO:0000313|Proteomes:UP000002593};
RN [1] {ECO:0000313|EMBL:ABM81475.1, ECO:0000313|Proteomes:UP000002593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5456 / JCM 9403 / PLM1-5
RC {ECO:0000313|Proteomes:UP000002593};
RX PubMed=17350933;
RA Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A.,
RA Awayez M., She Q., Garrett R.A., Klenk H.-P.;
RT "The genome of Hyperthermus butylicus: a sulfur-reducing, peptide
RT fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C.";
RL Archaea 2:127-135(2007).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 54 and uracil-55 in the psi GC loop of transfer RNAs.
CC {ECO:0000256|HAMAP-Rule:MF_01893}.
CC -!- CATALYTIC ACTIVITY: tRNA uridine(54) = tRNA pseudouridine(54).
CC {ECO:0000256|HAMAP-Rule:MF_01893}.
CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC {ECO:0000256|HAMAP-Rule:MF_01893}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC {ECO:0000256|HAMAP-Rule:MF_01893}.
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DR EMBL; CP000493; ABM81475.1; -; Genomic_DNA.
DR RefSeq; WP_011822793.1; NC_008818.1.
DR ProteinModelPortal; A2BNB4; -.
DR STRING; 415426.Hbut_1661; -.
DR EnsemblBacteria; ABM81475; ABM81475; Hbut_1661.
DR GeneID; 4782740; -.
DR KEGG; hbu:Hbut_1661; -.
DR eggNOG; arCOG01015; Archaea.
DR eggNOG; COG1258; LUCA.
DR HOGENOM; HOG000227928; -.
DR KO; K07583; -.
DR OMA; PQTRWPC; -.
DR OrthoDB; POG093Z05WB; -.
DR BioCyc; HBUT415426:GC56-1663-MONOMER; -.
DR Proteomes; UP000002593; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR HAMAP; MF_01893; Pus10_arch; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR005912; Pus10.
DR InterPro; IPR004114; THUMP_dom.
DR Pfam; PF02926; THUMP; 1.
DR SMART; SM00981; THUMP; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
DR PROSITE; PS51165; THUMP; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000002593};
KW Isomerase {ECO:0000256|HAMAP-Rule:MF_01893};
KW Reference proteome {ECO:0000313|Proteomes:UP000002593};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01893};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01893}.
FT DOMAIN 86 208 THUMP. {ECO:0000259|PROSITE:PS51165}.
FT ACT_SITE 265 265 Nucleophile. {ECO:0000256|HAMAP-
FT Rule:MF_01893}.
FT BINDING 333 333 Substrate. {ECO:0000256|HAMAP-
FT Rule:MF_01893}.
FT BINDING 405 405 Substrate. {ECO:0000256|HAMAP-
FT Rule:MF_01893}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 86 208 iprf:THUMP [T]
FT MYHIT 115 208 ismart:THUMP [T]
FT MYHIT 21 441 ihamap:Pus10_arch [T]
FT MYHIT 35 206 ipfam:THUMP [T]
SQ SEQUENCE 441 AA; 49834 MW; 96B98928B7BF793B CRC64;
MTGVHSDGVE AVITGIASKA LRLLEEIPLC DSCLGRMFGL LGRGFSNEER GRALKTLLVM
SLHARIREGD AGAREQLRRL APNIGELAAK LYEEVFGEKL VPNRCYICES RLQEMISLAV
KRARHMLEGL EFRGFIVAAR VNDEVRFRED EVKRRFSLEY AESIGSEVKR EVGKKLQQLI
GVKPDFEKPD VVVLVHVPEG RVELQVMPLL LRGWYWKLAR RVSQSAWVTR RGERRYPFSV
EDGLFILAEV FEADNIVIHA SGREDADARM LGTGRPFIVE AKRPRKRISG LAVAEVEANS
YAAGLVEYKL LSEARRSDVA SIKGETQHRA KLYKALVVVE RPISEDELKG LEEAFHMRSI
MQRTPRRVRH RRPDVVREKI VFSVKTRQLA DNVFEAIIHA EGGLYIKELV SGDGGDTRPS
FAEYFSARAY CAELDVLGVF S
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