ID A2BK15_HYPBU Unreviewed; 396 AA.
AC A2BK15;
DT 20-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 20-FEB-2007, sequence version 1.
DT 10-MAY-2017, entry version 66.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
DE EC=2.1.1.216 {ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(m(2,2)G26)dimethyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
GN Name=trm1 {ECO:0000256|HAMAP-Rule:MF_00290};
GN OrderedLocusNames=Hbut_0460 {ECO:0000313|EMBL:ABM80326.1};
OS Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Pyrodictiaceae; Hyperthermus.
OX NCBI_TaxID=415426 {ECO:0000313|EMBL:ABM80326.1, ECO:0000313|Proteomes:UP000002593};
RN [1] {ECO:0000313|EMBL:ABM80326.1, ECO:0000313|Proteomes:UP000002593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5456 / JCM 9403 / PLM1-5
RC {ECO:0000313|Proteomes:UP000002593};
RX PubMed=17350933;
RA Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A.,
RA Awayez M., She Q., Garrett R.A., Klenk H.-P.;
RT "The genome of Hyperthermus butylicus: a sulfur-reducing, peptide
RT fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C.";
RL Archaea 2:127-135(2007).
CC -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC a number of tRNAs using S-adenosyl-L-methionine as donor of the
CC methyl groups. {ECO:0000256|HAMAP-Rule:MF_00290}.
CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + guanine(26) in
CC tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in
CC tRNA. {ECO:0000256|HAMAP-Rule:MF_00290}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC methyltransferase superfamily. Trm1 family. {ECO:0000256|HAMAP-
CC Rule:MF_00290, ECO:0000256|PROSITE-ProRule:PRU00958,
CC ECO:0000256|SAAS:SAAS00541185}.
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DR EMBL; CP000493; ABM80326.1; -; Genomic_DNA.
DR RefSeq; WP_011821644.1; NC_008818.1.
DR ProteinModelPortal; A2BK15; -.
DR STRING; 415426.Hbut_0460; -.
DR EnsemblBacteria; ABM80326; ABM80326; Hbut_0460.
DR GeneID; 4782654; -.
DR KEGG; hbu:Hbut_0460; -.
DR eggNOG; arCOG01219; Archaea.
DR eggNOG; COG1867; LUCA.
DR HOGENOM; HOG000229931; -.
DR KO; K00555; -.
DR OMA; FYNPRMA; -.
DR OrthoDB; POG093Z0BNY; -.
DR BioCyc; HBUT415426:GC56-460-MONOMER; -.
DR Proteomes; UP000002593; Chromosome.
DR GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00290; tRNA_dimethyltr_TRM1; 1.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR022923; TRM1_arc_bac.
DR PANTHER; PTHR10631; PTHR10631; 1.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00308; TRM1; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000002593};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00290,
KW ECO:0000256|PROSITE-ProRule:PRU00958, ECO:0000256|SAAS:SAAS00423719,
KW ECO:0000313|EMBL:ABM80326.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002593};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00958,
KW ECO:0000256|SAAS:SAAS00423720};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00290,
KW ECO:0000256|PROSITE-ProRule:PRU00958, ECO:0000256|SAAS:SAAS00423718};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00290, ECO:0000256|PROSITE-
KW ProRule:PRU00958, ECO:0000256|SAAS:SAAS00423719,
KW ECO:0000313|EMBL:ABM80326.1};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00290, ECO:0000256|PROSITE-
KW ProRule:PRU00958, ECO:0000256|SAAS:SAAS00423721};
KW tRNA-binding {ECO:0000256|PROSITE-ProRule:PRU00958,
KW ECO:0000256|SAAS:SAAS00423720}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 8 393 ihamap:tRNA_dimethyltr_TRM1 [T]
FT MYHIT 8 391 iprf:SAM_MT_TRM1 [T]
FT MYHIT 35 391 ipfam:TRM [T]
SQ SEQUENCE 396 AA; 44965 MW; 4DF0E2A9CE1D4EF5 CRC64;
MDVGFPVRVV REGGVEIVVP DMDVYRRPNG VYEPAWAPVF YNPRMAFNRD IAVVFARTYA
RLRGLDKLVV VEPLAGSGVR AVRYAVEAGA IVFASDIDSD AVYLSRVNAE RNKVSERVRV
EKADANEFMA RLPRMGVKPT IIDIDPFGSP APFLDTAIQA LRPRGVLAVT ATDTAPLSGT
HPRALRRRYD VRPGRLAWEK EQAVRILAGY IIRRAAAHEY GVRILLAYYV DYYVRIYAEL
WRGAGRADKS LEQLGYGAYC YSCGYTSLAS NPLERCPYCQ AQLGIVGPLY IGKLCDESFI
EEMLREAEKM WDILAEPDRV VKLLEQLLKE CDIVKPYYRL DKLCSILRMN MPKMNTVVEE
LRNRGYNATR THFDPRGFKT NAPHWAVVKM LLETRR
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