ExPASy_SIB
MyHits
BLASTP/PSI-BLAST
user:
GUEST
width:
600
Tools
Search ...
Pattern Search
BLASTP/PSI-BLAST
PFSEARCH (profile)
HMMER3 (profile-HMM)
Motif Scan
Query ...
by Protein
by Motif
Align...
MAFFT
Profile Align
Classify ...
JACOP
Tools ...
Reformat MSA
Reformat SEQ
Dotlet
ProtoGene
Hub
Protein Hub
MSA Hub
Results
Stored results
Private area
Misc
Help Pages
Deprecated
Search...
HMMER2 (profile-HMM)
Align...
CLUSTALW
Translate ...
Translator
ESTScan
Privacy notice
This web interface to PSI-BLAST is meant to be used in conjunction with the MSA hub. To make a proficient use of this service, new users are warmly encouraged to read and try the
tutorial
.
Sequence Input
Protein Identifiers
or Protein Sequence
or MSA Input
examples
multiple FASTA format
identifier protein
FASTA format
raw format
>ur50:NRAM_I02A1 MNPNRKIITIGSICMVIGIVSLMLQIGNIISIWVSHSIQTENQHQAEPIS NTNFLTEKAVASVTLAGNSSLCPISGWAIHSKDNGIRIGSKGDVFVIREP FISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPY NSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWR NNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFKMEKGKVVKSVELD APNYHYEECSCYPDAGEVTCVCRDNWHGSNRPWVSFNQNLEYQIGYICSG VFGDNPRPNDGTGSCGPMSLNGAYGVKGFSFKYGNGVWIGRTKSTNSRSG FEMIWDPN
Mandatory parameters
seq_source
sw
- Swiss-Prot
bact
- Some complete proteomes from Bacteria
arch
- Some complete proteomes from Archaea
euk
- Some complete proteomes from Eukaryota
ur50
- UniRef50
ur50_win20
- UniRef50 window shuffled (w=20)
ecoli
- Escherichia coli K12 proteome
Show optional parameters
Optional parameters
Taxonomic restriction
(
NCBI taxonomic browser
)
- no restriction [all]-
Archaea [taxid:2157]
Bacteria (eubacteria) [taxid:2]
Eukaryota (eucaryotes) [taxid:2759]
Fungi [taxid:4751]
Stramenopiles [taxid:33634]
Metazoa [taxid:33208]
Arthropoda [taxid:6656]
Viridiplantae [taxid:33090]
Viruses [taxid:10239]
Homo_sapiens (human) [taxid:9606]
Mus_musculus (house mouse) [taxid:10090]
Xenopus (genus) [taxid:8353]
Caenorhabditis elegans [taxid:6239]
Drosophila_melanogaster (fruit fly) [taxid:7227]
Arabidopsis_thaliana (thale cress) [taxid:3702]
Saccharomyces cerevisiae [taxid:4932]
Schizosaccharomyces pombe [taxid:4896]
Search method
BLOSUM80 - 10 / 1
BLOSUM62 - 11 / 1
BLOSUM45 - 15 / 2
E-value report threshold
1e-6
1e-3
0.01
0.1
1
10
E-value inclusion threshold
1e-16
1e-9
1e-6
1e-5
1e-4
1e-3
0.01
0.1
1
Let PSI-blast cycle until convergence
Yes
No
Cluster matches at approx. identity level
100 % (all)
90 %
80 %
70 %
60 %
50 %