ExPASy_SIB
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BLASTP/PSI-BLAST
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This web interface to PSI-BLAST is meant to be used in conjunction with the MSA hub. To make a proficient use of this service, new users are warmly encouraged to read and try the
tutorial
.
Sequence Input
Protein Identifiers
or Protein Sequence
or MSA Input
examples
multiple FASTA format
identifier protein
FASTA format
raw format
>sw:ZN840_HUMAN MSLMGHGKVEMLLYAVPLLKAPNCLSSSMQLPHGGGRHQELVRFRDVAVV FSPEEWDHLTPEQRNLYKDVMLDNCKYLASLGNWTYKAHVMSSLKQGKEP WMMEREVTGDPCPACQPALATFRALNESGNAFRQSFHHGEYRTHRTFVQH YECDDCGMAFGHVSQLTGHQKIHKVGETHEYGENTRGFRHRSSFTMLQRI CTLYKHFECNQCGETFNRPSKVIQHQSMHSGLKPYKCDVCQKAFRFLSSL SIHQRFHVGNRVNLTRHQKTHTQRKPFSCNFCGKTFHRFSEKTQHLLIHT RKKYYTCNYCKKEFNPYSKFILHQRTHTGEKPHKCDVCEKSFKSISNLNK HQKTHTGEKPFSCNECKKTFAQRTDLARHQQIHTGKKSFICSSCKKTFVR LSDLTQHKGTHTGERPYQCTTCEKAFKYRSNFTKHQKTHSIGRPFACNEC GKTYRLNWELNQHKKIHTGEKPYECGECGKRFNNNSNLNKHKKIHTGEKH FVCNQCGKAFSLNSKLSRHQRTHNKKENSSKSVSNLNKHQKTHAGEKPFP CNECKKAFAQRMDLARHQQIHTGRKPFICSSCKKTFVRLSDLTQHKGTHT GERPYQCTTCEKAFKYQSNFTKHQKTHSIGRPFTCNECGKTFRLNWKLNQ HKKIHTGEKPYECGECGKCFNNNSNLSKHKKIHTGEKHFVCNQCGKAFSL NSKLSRHQITHNKKKP
Mandatory parameters
seq_source
sw
- Swiss-Prot
bact
- Some complete proteomes from Bacteria
arch
- Some complete proteomes from Archaea
euk
- Some complete proteomes from Eukaryota
ur50
- UniRef50
ur50_win20
- UniRef50 window shuffled (w=20)
ecoli
- Escherichia coli K12 proteome
Show optional parameters
Optional parameters
Taxonomic restriction
(
NCBI taxonomic browser
)
- no restriction [all]-
Archaea [taxid:2157]
Bacteria (eubacteria) [taxid:2]
Eukaryota (eucaryotes) [taxid:2759]
Fungi [taxid:4751]
Stramenopiles [taxid:33634]
Metazoa [taxid:33208]
Arthropoda [taxid:6656]
Viridiplantae [taxid:33090]
Viruses [taxid:10239]
Homo_sapiens (human) [taxid:9606]
Mus_musculus (house mouse) [taxid:10090]
Xenopus (genus) [taxid:8353]
Caenorhabditis elegans [taxid:6239]
Drosophila_melanogaster (fruit fly) [taxid:7227]
Arabidopsis_thaliana (thale cress) [taxid:3702]
Saccharomyces cerevisiae [taxid:4932]
Schizosaccharomyces pombe [taxid:4896]
Search method
BLOSUM80 - 10 / 1
BLOSUM62 - 11 / 1
BLOSUM45 - 15 / 2
E-value report threshold
1e-6
1e-3
0.01
0.1
1
10
E-value inclusion threshold
1e-16
1e-9
1e-6
1e-5
1e-4
1e-3
0.01
0.1
1
Let PSI-blast cycle until convergence
Yes
No
Cluster matches at approx. identity level
100 % (all)
90 %
80 %
70 %
60 %
50 %